Of course you should check Florida law. However, Florida rules probably follow that of most states, which follow the federal rules of civil procedure. Put very simply, if a person is not a party to the case, like the spouse, she does not have to come no matter what she agreed if you did not subpoena her. You need to subpoena a non-party. A party (your ex) only requires a written notice of deposition. You cannot do anything to the spouse if she does not come if you did not subpoena her. The previous notice to her does not count, in other words, since you did not do it correctly. Do it again. And when you do, you need to look up the Florida rules on subpoenas to see how to serve the subpoena, and who can serve it. Otherwise, you may be doing it wrong again. Then, if you do it correctly (probably at least 3 days before the deposition), if she doesn't appear, you can ask the court to order her to appear or hold her in contempt. All of these rules are in the Florida statutes, go to a law library and look them up. Your local courthouse probably has a law library, but the Florida statutes are also probably on the internet.